The study of the Oxytropis kansuensis-induced apoptotic pathway in the cerebrum of SD rats

BMC Veterinary Research

Bao-yu Zhao¹  Wen-long Wang¹  Shan-shan Wang¹  Liang Zhang¹  Hao Lu¹ 

[1] College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, People’s Republic of China

关键词: Apoptotic pathway;    Cerebrum;    SD rats;    Swainsonine;    Oxytropis kansuensis;   

Others  :  1119421
DOI  :  10.1186/1746-6148-9-217

 received in 2013-06-06, accepted in 2013-10-18,  发布年份 2013

【 摘 要 】

Background

Locoweeds cause significant livestock poisoning and economic loss all over the world. Animals can develop locoism, a chronic neurological disease, after grazing on locoweeds. Oxytropis kansuensis is a variety of locoweed that contains swainsonine as its main toxic ingredient. The purpose of this study was to investigate the apoptotic pathway induced in the cerebrum by swainsonine.

Results

Twenty-four Sprague-Dawley rats were randomly divided into four groups (experimental groups I, II, III and a control group) and 6 SD rats of each group were feed in 3 cages separately. Rats were penned as groups and fed with feeds containing 15% (SW content 0.03‰), 30% (SW content 0.06‰), or 45% (SW content 0.09‰) O. kansuensis for experimental groups I, II, and III, respectively, or complete feed in the case of the control group. One hundred and nineteen days after poisoning, and all rats showed neurological disorders at different degrees, which were considered to be successful established a chronic poisoning model of O. kansuensis. rats were sacrificed and the expression of Fas, FasL, Bcl-2, Bax as well as cleaved caspase-3, -8 and -9 proteins in brain tissues were detected by Western blot. The results showed that SW treatment up-regulated Fas and Fas ligand (FasL) (P < 0.05), and that there was an increase in Bax and a decrease in Bcl-2 protein (P < 0.01). Moreover, SW treatment significantly increases the activation of caspase-3, 8 and -9, the key effectors in apoptosis pathway (P < 0.01).

Conclusion

Our data suggest that SW induces apoptosis in cells of the brain through death receptor and mitochondria-mediated, caspase-dependent apoptotic pathways in the brain tissue of SD rats.

【 授权许可】

   
2013 Lu et al.; licensee BioMed Central Ltd.

【 图 表 】

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