Biosynthesis of the major scent components 3,5‐dimethoxytoluene and 1,3,5‐trimethoxybenzene by novel rose O‐methyltransferases

FEBS Letters

Dumas, Christian²  Hugueney, Philippe²  Scalliet, Gabriel²  Magnard, Jean-Louis¹  Channelière, Stéphanie²  Vergne, Philippe²  Journot, Noellie²  Jullien, Frédéric¹  Bendahmane, Mohammed²  Baudino, Sylvie¹  Cock, J.Mark² 

[1] Laboratoire de Biotechnologies Végétales Appliquées aux Plantes Aromatiques et Médicinales, Université Jean Monnet, 23, rue du Dr. Michelon, 42023 Saint-Etienne Cedex 2, France;Reproduction et Développement des Plantes Laboratory, Ecole Normale Supérieure de Lyon, UMR 5667 CNRS-INRA-ENSL-UCBL, 46 allée d'Italie, 69364 Lyon Cedex 07, France

关键词: Flower;    O-Methyltransferase;    Petal;    Rose;    Scent;    Rosa spp;    AdoMet;    S-adenosyl-L-methionine;    CVOMT;    chavicol OMT;    DMT;    3;    5-dimethoxytoluene;    EOMT;    eugenol OMT;    EST;    expressed sequence tag;    GST;    glutathione S-transferase;    IOMT;    isoflavone OMT;    OMT;    O-methyltransferase;    OOMT;    orcinol OMT;    RT-PCR;    reverse transcriptase-polymerase chain reaction;    TLC;    thin layer chromatography;    TMB;    1;    3;    5-trimethoxybenzene;   

DOI  :  10.1016/S0014-5793(02)02956-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In Chinese rose species and in many modern varieties, two methylated phenolic derivatives, 3,5-dimethoxytoluene and 1,3,5-trimethoxybenzene, are major scent components. We show that cell-free extracts of rose petals catalyse the synthesis of 3,5-dimethoxytoluene and 1,3,5-trimethoxybenzene by methylation of precursor molecules. An expressed sequence tag approach was used to identify four highly similar O-methyltransferase sequences expressed specifically in petals and anthers. Thin layer chromatography analysis showed that the activities of these enzymes with different substrates and the proportions of reaction products produced closely mimicked those observed using cell-free petal extracts, indicating that orcinol O-methyltransferases are responsible for the biosynthesis of 3,5-dimethoxytoluene and 1,3,5-trimethoxybenzene from un-methylated precursors in this organ.

【 授权许可】

Unknown