Analysis of gene expression in rose petals using expressed sequence tags

FEBS Letters

Szecsi, Judit¹  Dumas, Christian¹  Hugueney, Philippe¹  Scalliet, Gabriel¹  Rivière, Stéphane¹  Channelière, Stéphanie¹  Vergne, Philippe¹  Dolle, Caroline¹  Jullien, Frédéric²  Bendahmane, Mohammed¹  Cock, J.Mark¹ 

[1] Reproduction et Développement des Plantes, UMR 5667 CNRS-INRA-ENSL, Ecole Normale Supérieure de Lyon, 46 allée d'Italie, 69364 Lyon Cedex 07, France;Laboratoire de Biotechnologies Végétales appliquées aux Plantes Aromatiques et Médicinales, Université Jean Monnet, 23, rue du Dr Michelon, 42023 Saint-Etienne Cedex 2, France

关键词: Expressed sequence tag;    Petal;    Rose;    Scent;    Senescence;    Rosa chinensis;    EF1α;    elongation factor 1α;    EST;    expressed sequence tag;    GAPDH;    glyceraldehyde-3-phosphate dehydrogenase;    RT-PCR;    reverse transcriptase-polymerase chain reaction;   

DOI  :  10.1016/S0014-5793(02)02413-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Single-pass sequences were obtained from the 5′-ends of a total of 1794 rose petal cDNA clones. Cluster analysis identified 242 groups of sequences and 635 singletons indicating that the database represents a total of 877 genes. Putative functions could be assigned to 1151 of the transcripts. Expression analysis indicated that transcripts of several of the genes identified accumulated specifically in petals and stamens. The cDNA library and expressed sequence tag database described here represent a valuable resource for future research aimed at improving economically important rose characteristics such as flower form, longevity and scent.

【 授权许可】

Unknown