Factor Xa induces mitogenesis of coronary artery smooth muscle cell via activation of PAR‐2

FEBS Letters

Hwang, Ki-Chul¹  Kim, Doo-Sik²  Koo, Bon-Hun²  Chung, Kwang-Hoe¹ 

[1] Cardiovascular Research Institute, Yonsei University College of Medicine, Seoul 120-752, South Korea;Department of Biochemistry, College of Science, and Bioproducts Research Center, Yonsei University, Seoul 120-749, South Korea

关键词: Coronary artery smooth muscle cell;    Factor Xa;    Protease-activated receptor-2;   

DOI  :  10.1016/S0014-5793(02)02948-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Factor Xa-induced stimulation of coronary artery smooth muscle cells (CASMC) was investigated by analyzing [³H]thymidine incorporation, cell proliferation, and ERK-1/2 activation. Exposure of the cells to factor Xa evoked a time-dependent activation of ERK-1/2 with increased [³H]thymidine incorporation and cell proliferation. The factor Xa-induced ERK-1/2 activation was not desensitized by preincubation of the cells with thrombin. However, ERK-1/2 activation was markedly attenuated by prior exposure of the cells to protease-activated receptor-2 (PAR-2) activating peptide, SLIGKV. The mitogenic effect of factor Xa was significantly reduced in the presence of anti-PAR-2 monoclonal antibody. Several lines of experimental evidence indicate that factor Xa-induced mitogenesis of CASMC is a cellular process mediated by PAR-2 activation.

【 授权许可】

Unknown