Protein disulfide isomerase of Toxoplasma gondii is targeted by mucosal IgA antibodies in humans

FEBS Letters

Meek, Bob³  Peek, Ron³  Back, Jaap Willem¹  Klaren, Vincent N.A³  Speijer, Dave² 

[1]Swammerdam Institute for Life Sciences, Mass Spectrometry Group, University of Amsterdam, Amsterdam, The Netherlands

[2]Department of Biochemistry, University of Amsterdam, Amsterdam, The Netherlands

[3]Department of Molecular Immunology, the Netherlands Ophthalmic Research Institute, Meibergdreef 47, 1105 BA Amsterdam, The Netherlands

关键词: Human;    Parasitic protozoan;    Mucosa;    Natural antibody;    IgA;    Protein disulfide isomerase;    Mass spectrometry;    Toxoplasma;   

DOI  :  10.1016/S0014-5793(02)02911-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mass spectrometric analysis identified a 49 kDa antigen from Toxoplasma gondii as protein disulfide isomerase (PDI). This antigen is generally recognized by IgA in tears of healthy humans. We determined the complete open reading frame and expressed PDI recombinantly. Recombinant PDI was recognized by IgA in human tears known to contain antibodies specific for the 49 kDa antigen. High expression level and similarity to other protozoan PDIs suggest that T. gondii PDI might be a suitable target for recently described anti-protozoan drugs. PDI-specific antibodies clearly constitute part of the mucosal antibody repertoire possibly involved in defence against parasites.

【 授权许可】

Unknown