Overexpression of human metallothionein‐III prevents hydrogen peroxide‐induced oxidative stress in human fibroblasts

FEBS Letters

Jeong, Hye Gwang³  You, Ho Jin¹  Lee, Kyung Jin² 

[1] Department of Pharmacology, School of Medicine, Chosun University, Kwangju, South Korea;Department of Biology, Chonnam National University, Kwangju, South Korea;Department of Pharmacy, College of Pharmacy, Chosun University, 375 Seosuk-dong, Kwangju 501-759, South Korea

关键词: Metallothionein-III;    Hydrogen peroxide;    Antioxidant;   

DOI  :  10.1016/S0014-5793(02)02870-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Metallothioneins (MT) are ubiquitous low molecular weight metal binding proteins that may act as antioxidants. In the present study, the cloned human MT-III coding region was permanently transfected into GM00637 cells in order to investigate the antioxidative effects of this brain-specific MT isoform. GM00637/MT-III cells overexpressed MT-III mRNA versus pcDNA3 plasmid-transfected control cells (GM00637). When challenged with H₂O₂, the GM00637/MT-III cells displayed significantly more resistance than the GM00637 cells, as determined by cell cytotoxicity, lactate dehydrogenase leakage, and lipid peroxidation. In addition, the GM00637/MT-III cells were highly protected from the H₂O₂-induced production of reactive oxygen species and DNA damage. These results directly support the antioxidative role of MT-III, and demonstrate MT-III can scavenge free oxygen radicals and protect cells from oxidative stress.

【 授权许可】

Unknown