Halocidin: a new antimicrobial peptide from hemocytes of the solitary tunicate, Halocynthia aurantium

FEBS Letters

Jang, Woong Sik²  Lee, In Hee²  Kim, Kyu Nam²  Nam, Myung Hee¹  Lee, Young Shin² 

[1] Korea Basic Science Institute, Anam-Dong Sungbuk-Gu, Seoul, South Korea;Department of Life Science, Hoseo University, 29-1 Sechuli, Baebang-Myun Asan City, Choongnam-Do 336-795, South Korea

关键词: Antimicrobial peptide;    Tunicate;    Hemocyte;    Heterodimer;    Antibiotic-resistant bacterium;   

DOI  :  10.1016/S0014-5793(02)02827-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

From hemocytes of the tunicate Halocynthia aurantium we purified a new antimicrobial peptide named halocidin. The native peptide had a mass of 3443 Da and comprised two different subunits containing 18 amino acid residues (WLNALLHHGLNCAKGVLA) and 15 residues (ALLHHGLNCAKGVLA), which were linked covalently by a single cystine disulfide bond. Two different monomers were separately synthesized and used to make three additional isoforms (15 residue homodimer, 18 residue homodimer, heterodimer). In antimicrobial assays performed with synthetic peptides of halocidin, it was confirmed that congeners of the 18 residue monomer were more active than those of the 15 residue monomer against methicillin-resistant Staphylococcus aureus and multidrug-resistant Pseudomonas aeruginosa.

【 授权许可】

Unknown