Isolation of up‐ or down‐regulated genes in PPARγ‐expressing NIH‐3T3 cells during differentiation into adipocytes

FEBS Letters

Nishizuka, Makoto¹  Arimoto, Emi¹  Imagawa, Masayoshi²  Nishihara, Tsutomu¹  Okuno, Masaaki¹ 

[1]Laboratory of Environmental Biochemistry, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-Oka, Suita, Osaka 565-0871, Japan

[2]Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya, Aichi 467-8603, Japan

关键词: Peroxisome proliferator-activated receptor γ;    Adipocyte differentiation;    Gene expression;    Obesity;    Subtraction method;    PPARγ;    peroxisome proliferator-activated receptor γ;    aP2;    adipocyte fatty acid binding protein;    CRABP-1;    cellular retinoic acid binding protein 1;    CS;    calf serum;    Dex;    dexamethazone;    DMEM;    Dulbecco's modified Eagle's medium;    FBS;    fetal bovine serum;    IBMX;    isobutyl methyxanthine;    Ins;    insulin;    PCR;    polymerase chain reaction;    TSP1;    thrombospondin 1;   

DOI  :  10.1016/S0014-5793(02)02720-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Adipocyte differentiation is a complex process in which the expression of many transcription factors and adipocyte-specific genes is regulated under a strict program. The peroxisome proliferator-activated receptor γ (PPARγ), a member of the steroid/thyroid nuclear hormone receptor superfamily of ligand-activated transcription factors, is an important regulator of adipocyte gene expression and differentiation. In this study, we tried to identify downstream target genes of PPARγ, by using PPARγ-expressing cells and a subtractive cloning strategy, and isolated cDNA clones which were up-regulated or down-regulated by PPARγ. Northern blot analyses revealed that the expression levels of the aldehyde dehydrogenase-2-like, type VI collagen alpha 3 subunit, cellular retinoic acid binding protein 1 and thrombospondin 1 are changed during the differentiation of mouse 3T3-L1 preadipocyte cells, indicating that these genes might be downstream targets of PPARγ in adipocyte differentiation.

【 授权许可】

Unknown