Impact of Metabolic Regulators on the Expression of the Obesity Associated Genes FTO and NAMPT in Human Preadipocytes and Adipocytes

PLoS One

Antje Körner¹  Maria Schönberg¹  Wieland Kiess²  Petra Büttner²  Kathrin Landgraf²  Daniela Friebe²  Falk Bernhard²  Dennis Löffler² 

[1] Leipzig University Medical Center, IFB Adiposity Diseases, Leipzig, Germany;University Hospital for Children and Adolescents, University of Leipzig, Leipzig, Germany

关键词: Adipocytes;    Glucose metabolism;    Adipocyte differentiation;    Glucose;    Gene expression;    Isoproterenol;    Insulin;    Regulator genes;   

DOI  :  10.1371/journal.pone.0019526

学科分类：医学（综合）

来源: Public Library of Science

【 摘 要 】

Background FTO and NAMPT/PBEF/visfatin are thought to play a role in obesity but their transcriptional regulation in adipocytes is not fully understood. In this study, we evaluated the transcriptional regulation of FTO and NAMPT in preadipocytes and adipocytes by metabolic regulators. Methodology and Principal Findings We assessed FTO mRNA expression during human adipocyte differentiation of Simpson-Golabi-Behmel syndrome (SGBS) cells and primary subcutaneous preadipocytes in vitro and evaluated the effect of the metabolic regulators glucose, insulin, dexamethasone, IGF-1 and isoproterenol on FTO and NAMPT mRNA expression in SGBS preadipocytes and adipocytes. FTO mRNA levels were not significantly modulated during adipocyte differentiation. Also, metabolic regulators had no impact on FTO expression in preadipocytes or adipocytes. In SGBS preadipocytes NAMPT expression was more than 3fold induced by dexamethasone and isoproterenol and 1.6fold by dexamethasone in adipocytes. Complete glucose restriction caused an increase in NAMPT mRNA expression by more than 5fold and 1.4fold in SGBS preadipocytes and adipocytes, respectively. Conclusion FTO mRNA expression is not significantly affected by differentiation or metabolic regulators in human adipocytes. The stimulation of NAMPT expression by dexamethasone, isoproterenol and complete glucose restriction may indicate a regulation of NAMPT by metabolic stress, which was more pronounced in preadipocytes compared to mature adipocytes.

【 授权许可】

CC BY