FEBS Letters | |
Metabolic engineering of xanthophyll content in tomato fruits | |
Camara, Bilal1  Dharmapuri, Sridhar2  Rosati, Carlo2  Aquilani, Riccardo2  Bouvier, Florence1  Giuliano, Giovanni2  Pallara, Patrizia2  | |
[1] CNRS, Plant Molecular Biology Institute, 12 rue du Général Zimmer, 67084 Strasbourg Cedex, France;ENEA, Casaccia Research Center, P.O. Box 2400, Rome 00100AD, Italy | |
关键词: β-Carotene; Zeaxanthin; β-Cryptoxanthin; | |
DOI : 10.1016/S0014-5793(02)02699-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Ripe tomato fruits accumulate significant amounts of the linear carotene lycopene, but only trace amounts of xanthophylls (oxygenated carotenoids). We overexpressed the lycopene β-cyclase (b-Lcy) and β-carotene hydroxylase (b-Chy) genes under the control of the fruit-specific Pds promoter. Transgene and protein expression was followed through semi-quantitative reverse transcription-PCR, Western blotting, and enzyme assays. Fruits of the transformants showed a significant increase of β-carotene, β-cryptoxanthin and zeaxanthin. The carotenoid composition of leaves remained unaltered. The transgenes and the phenotype are inherited in a dominant Mendelian fashion. This is the first example of successful metabolic engineering of xanthophyll content in tomato fruits.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020311801ZK.pdf | 230KB | download |