Design of a chimeric promoter induced by pro‐inflammatory mediators in articular chondrocytes

FEBS Letters

Meynier de Salinelles, Véronique¹  Berenbaum, Francis¹  Olivier, Jean-Luc¹  Massaad, Charbel¹  Béréziat, Gilbert¹  Salvat, Colette¹  Jacques, Claire¹  Raymondjean, Michel¹ 

[1] UMR Physiologie et Physiopathologie, Université Pierre et Marie Curie, 7 quai Saint Bernard, 75252 Paris, Cedex 05, France

关键词: Type IIA secreted phospholipase A2;    Peroxisome proliferator-activated receptor;    Interleukin-1β;    Transcription;    Chondrocyte;    sPLA2-IIA;    type IIA secreted phospholipase A2;    PPAR;    peroxisome proliferator-activated receptor;    IL-1β;    interleukin-1β;    PGJ2;    15-deoxy Δ12;    14 prostaglandin J2;    NSAID;    non-steroidal anti-inflammatory drug;    DR1;    direct repeat 1;   

DOI  :  10.1016/S0014-5793(02)02645-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have designed a chimeric promoter that can be stimulated by various pro-inflammatory mediators and so drive the expression of therapeutic genes under inflammatory conditions. The promoter has two parts, the [−247/+20] fragment of the human type IIA secreted phospholipase A2 gene promoter, which is stimulated by the pro-inflammatory cytokine interleukin-1β (IL-1β), and a double peroxisome proliferator-activated receptor response element that is activated by some eicosanoids and by non-steroidal anti-inflammatory drugs (NSAIDs). Transfection experiments using rabbit articular chondrocytes in primary culture showed that this chimeric promoter produced a low basal activity and was induced by NSAIDs, WY-14643, IL-1β, and 15-deoxy Δ^12,14 prostaglandin J2. The latter two compounds stimulated the promoter synergistically.

【 授权许可】

Unknown