A metacaspase of Trypanosoma brucei causes loss of respiration competence and clonal death in the yeast Saccharomyces cerevisiae

FEBS Letters

Duszenko, Michael¹  Kubata, Bruno K.²  Szallies, Alexander¹ 

[1] Verfügungsgebäude der Universität Tübingen, Auf der Morgenstelle 15, 72076 Tübingen, Germany;Department of Molecular Behavioral Biology, Osaka Bioscience Institute, Osaka 565-0874, Japan

关键词: Metacaspase;    Cysteine protease;    WW domain;    Mitochondrion;    Saccharomyces cerevisiae;    Trypanosoma brucei;    GFP;    green fluorescent protein;    GPI;    glycosylphosphatidylinositol;    PCD;    programmed cell death;    RT-PCR;    reverse transcription based polymerase chain reaction;    WW domain;    domain with two conserved tryptophan (W) residues;   

DOI  :  10.1016/S0014-5793(02)02608-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Metacaspases constitute a new group of cysteine proteases homologous to caspases. Heterologous expression of Trypanosoma brucei metacaspase TbMCA4 in the budding yeast Saccharomyces cerevisiae resulted in growth inhibition, mitochondrial dysfunction and clonal death. The metacaspase orthologue of yeast, ScMCA1 (YOR197w), exhibited genetic interaction with WWM1 (YFL010c), which encodes a small WW domain protein. WWM1 overexpression resulted in growth arrest and clonal death, which was suppressed by concomitant overexpression of ScMCA1. GFP-fusion reporters of WWM1, ScMCA1 and TbMCA4 localized to the nucleus. Taken together, we suggest that metacaspases may play a role in nuclear function controlling cellular proliferation coupled to mitochondrial biogenesis.

【 授权许可】

Unknown