FEBS Letters | |
Yeast Npi3/Bro1 is involved in ubiquitin‐dependent control of permease trafficking | |
André, Bruno1  Nikko, Elina1  Marini, Anne-Marie1  Springael, Jean-Yves1  | |
[1] Laboratoire de Physiologie Cellulaire, Institut de Biologie et de Médecine Moléculaires, Université Libre de Bruxelles, P.O. Box 300, Rue des Professeurs Jeener et Brachet 12, 6041 Gosselies, Belgium | |
关键词: Npi3/Bro1; Ubiquitin; Permease; Traffic; Yeast; | |
DOI : 10.1016/S0014-5793(02)02586-3 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The membrane traffic and stability of the general amino acid permease Gap1 of Saccharomyces cerevisiae are under nitrogen control. Addition of a preferential nitrogen source such as ammonium to cells growing on a poor nitrogen source induces internalization of the permease and its subsequent degradation in the vacuole. This down-regulation requires ubiquitination of Gap1 through a process involving ubiquitin ligase Npi1/Rsp5, ubiquitin hydrolase Npi2/Doa4, and Bul1/2, two Npi1/Rsp5 interacting proteins. Here we report that yet another protein, Npi3, is involved in the regulation of Gap1 trafficking. We show that Npi3 is required for NH4 +-induced down-regulation of Gap1, and particularly for efficient ubiquitination of the permease. Npi3 plays a pleiotropic role in permease down-regulation, since it is also involved in ubiquitination and stress-induced down-regulation of the uracil permease Fur4 and in glucose-induced degradation of hexose transporters Hxt6/7. We further provide evidence that Npi3 is required for direct vacuolar sorting of neosynthesized Gap1 permease as it occurs in npr1 mutant cells. NPI3 is identical to BRO1, a gene encoding a protein of unknown biochemical function and recently proposed to be involved in protein turnover. Npi3/Bro1 homologues include fungal proteins required for proteolytic cleavage of zinc finger proteins and the mouse Aip1 protein involved in apoptosis. We propose that proteins of the Npi3/Bro1 family, including homologues from higher species, may play a conserved role in ubiquitin-dependent control of membrane protein trafficking.
【 授权许可】
Unknown
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