Phytanic acid, but not pristanic acid, mediates the positive effects of phytol derivatives on brown adipocyte differentiation

FEBS Letters

Giralt, Marta¹  Iglesias, Roser¹  Villarroya, Francesc¹  Schluter, Agatha¹ 

[1] Departament de Bioquı́mica i Biologia Molecular, Universitat de Barcelona, Avinguda Diagonal 645, E-08028 Barcelona, Spain

关键词: Phytol;    Phytanic acid;    Brown adipose tissue;    Cell differentiation;    PhyH;    phytanoyl-CoA hydroxylase;    RXR;    retinoid X receptor;    PPAR;    peroxisome proliferator-activated receptor;    FCS;    fetal calf serum;    DMEM;    Dulbecco's modified Eagle's medium;    COT;    carnitine octanoyl transferase;    UCP1;    uncoupling protein-1;    CAT;    chloramphenicol acetyltransferase;   

DOI  :  10.1016/S0014-5793(02)02583-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The phytol derivatives phytanic acid and pristanic acid may activate nuclear hormone receptors and influence gene expression and cell differentiation. Phytanic acid induces brown adipocyte differentiation. It was determined that brown fat and brown adipocytes are sites of high gene expression of phytanoyl-CoA hydroxylase, the enzyme required for initiation of peroxisomal α-oxidation of phytanic acid. However, the effects of phytanic acid were not mediated by its α-oxidation product pristanic acid, which did not promote brown adipocyte differentiation or stimulate transcription of the uncoupling protein-1 gene. Moreover, acute cold exposure of mice caused a dramatic mobilization of the phytanic acid stores in brown adipose tissue thus suggesting that a high local exposure to phytanic acid in brown fat may contribute to signalling adaptive changes in the tissue in response to thermogenic activation.

【 授权许可】

Unknown