Trans‐translation mediated by Bacillus subtilis tmRNA

FEBS Letters

Takada, Kazuma²  Lee, SungGa¹  Muto, Akira²  Tadaki, Toshimasa²  Ito, Ken-ichi²  Himeno, Hyouta² 

[1] Department of Biochemistry and Biotechnology, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki 036-8561, Japan;Department of Biology, Faculty of Science, Hirosaki University, Hirosaki 036-8561, Japan

关键词: Bacillus subtilis tmRNA;    Trans-translation;    Tag-peptide;    SsrA;    S1;   

DOI  :  10.1016/S0014-5793(02)02561-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Trans-translation, in which a ribosome switches between translation of an mRNA and a tmRNA, produces a chimera polypeptide of an N-terminal truncated polypeptide and a C-terminal tag-peptide encoded by tmRNA. One of the tmRNA binding proteins, a ribosomal protein S1, has not been found in a group of Gram-positive bacteria. In this study, the trans-translation reaction with tmRNA from Bacillus subtilis belonging to this group was examined. When a truncated gene lacking a termination codon was expressed in B. subtilis, a 15-amino acid tag-peptide derived from tmRNA was identified in the C-termini of the trans-translation products. An identical tag-peptide was also found at the C-termini of the products from a truncated gene, when it was coexpressed with B. subtilis tmRNA in Escherichia coli. B. subtilis tmRNA was functional, although much less efficiently, in the in vitro poly(U)-dependent tag-peptide synthesis system of E. coli. A comparison of two bacterial tmRNAs suggests that the rule for determining the tag-initiation point on tmRNA may be the same in Gram-positive and Gram-negative bacteria.

【 授权许可】

Unknown