A new RNase‐based immunoconjugate selectively cytotoxic for ErbB2‐overexpressing cells

FEBS Letters

De Lorenzo, Claudia¹  Piccoli, Renata¹  D'Alessio, Giuseppe¹  Nigro, Alessandra¹ 

[1] Dipartimento di Chimica Biologica, Università di Napoli Federico II, Via Mezzocannone 16, 80134 Naples, Italy

关键词: Immunoconjugate;    RNase;    ErbB2;    Antitumor;    Immunotherapy;    scFv;    single-chain Fv fragment;    HP-RNase;    human pancreas RNase;    IR;    immunoRNase;    or RNase-based immunoconjugate;    ERB-HPR;    immunoRNase made up of an anti-ErbB2 scFv fused to HP-RNase;   

DOI  :  10.1016/S0014-5793(02)02527-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report a new tumor-directed immunoRNase, a chimeric protein made up of an antibody fragment (single-chain Fv fragment) directed to ErbB2, a cell surface receptor, and a non-toxic, human ribonuclease, which upon cell internalization becomes cytotoxic. The immunoRNase is active as a ribonuclease, specifically binds and selectively kills ErbB2-positive cells. ErbB2 is one of the most specific tumor-associated antigens identified so far, overexpressed on tumor cells of different origin. Its choice as target antigen and that of a non-toxic, human RNase as the killer moiety makes this immunoRNase a new, potentially attractive anticancer agent.

【 授权许可】

Unknown