Reactive oxygen species inhibit the succinate oxidation‐supported generation of membrane potential in wheat mitochondria

FEBS Letters

Passarella, Salvatore²  Di Fonzo, Natale¹  Pastore, Donato²  Laus, Maura Nicoletta² 

[1]Istituto Sperimentale per la Cerealicoltura, Sezione Operativa di Foggia, S.S. 16 Km 675, 71100 Foggia, Italy

[2]Dipartimento di Scienze Animali, Vegetali e dell'Ambiente, Facoltà di Agraria, Università del Molise, Via De Sanctis, 86100 Campobasso, Italy

关键词: Durum wheat mitochondria;    Reactive oxygen species;    Succinate carrier;    Electrical membrane potential;    BSA;    bovine serum albumin;    DWM;    durum wheat mitochondria;    ΔΨ;    electrical membrane potential;    EDTA;    ethylenediaminetetraacetic acid;    FCCP;    carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone;    NEM;    N-ethylmaleimide;    PmitoKATP;    plant mitochondrial potassium channel;    PUMP;    plant uncoupling mitochondrial protein;    PVP;    polyvinylpyrrolidone;    ROS;    reactive oxygen species;    Tris;    tris(hydroxymethyl)-aminomethane;   

DOI  :  10.1016/S0014-5793(02)02454-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In order to gain a first insight into the effects of reactive oxygen species (ROS) on plant mitochondria, we studied the effect of the ROS producing system consisting of xanthine plus xanthine oxidase on the rate of membrane potential (ΔΨ) generation due to either succinate or NADH addition to durum wheat mitochondria as monitored by safranin fluorescence. We show that the early ROS production inhibits the succinate-dependent, but not the NADH-dependent, ΔΨ generation and oxygen uptake. This inhibition appears to depend on the impairment of mitochondrial permeability to succinate. It does not involve mitochondrial thiol groups sensitive to either mersalyl or N-ethylmaleimide and might involve both protein residues and/or membrane lipids, as suggested by the mixed nature. We propose that, during oxidative stress, early generation of ROS can affect plant mitochondria by impairing metabolite transport, thus preventing further substrate oxidation, ΔΨ generation and consequent large-scale ROS production.

【 授权许可】

Unknown