Inhibition of mitochondrial and plastid activity of Plasmodium falciparum by minocycline

FEBS Letters

Katakura, Ken¹  Lin, Qinghua¹  Suzuki, Mamoru¹ 

[1]Department of Parasitology, Gunma University School of Medicine, 3-39-22 Showa-machi, Maebashi 371-8511, Japan

关键词: Minocycline;    Mitochondrion;    Plastid;    Reverse transcription-polymerase chain reaction;    Plasmodium falciparum;    COI;    cytochrome c oxidase subunit I;    Cyb;    apocytochrome b;    MSA1;    merozoite surface antigen 1;    RT-PCR;    reverse transcriptation-polymerase chain reaction;    rpoB/C;    RNA polymerase subunits B and C;    ssu rRNA;    small subunit ribosomal RNA;   

DOI  :  10.1016/S0014-5793(02)02437-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We previously reported the superior effect of minocycline against drug-resistant Plasmodium falciparum in vitro. Here, we report that RT-PCR for falciparum parasites treated with minocycline revealed reduced levels of RNA transcripts of the mitochondrion-encoded genes such as the COI and Cyb genes, as well as the plastid-encoded RNA polymerase subunit (rpoB/C) gene. However, we detected no apparent effects of the antibiotic on the transcription of merozoite surface antigen and small subunit rRNA genes encoded by the nucleus. In addition, treatment with chloroquine and pyrimethamine showed no substantial reduction of any RT-PCR products. These findings suggest that tetracycline antibiotics selectively inhibit both mitochondrial and plastid activity.

【 授权许可】

Unknown