Cloning and functional expression of a novel isoform of ROMK inwardly rectifying ATP‐dependent K+ channel, ROMK6 (Kir1.1 f)

FEBS Letters

Isomoto, Shojiro¹  Matsuzawa, Yuji²  Matsumoto, Shigeto¹  Horio, Yoshiyuki¹  Takemura-Kameda, Kaoru²  Yamada, Mitsuhiko¹  Kurachi, Yoshihisa¹  Yamashita, Shizuya²  Kondo, Chikako¹ 

[1] Department of Pharmacology II, Faculty of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565, Japan;Internal Medicine II, Faculty of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565, Japan

关键词: Cloning;    Inwardly rectifying K+ channel;    ROMK channel;    Splice variant;    Reverse transcription-polymerase chain reaction;    Xenopus oocyte;   

DOI  :  10.1016/S0014-5793(96)01302-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have identified from rat kidney a novel isoform of ROMK/Kir1.1, designated ROMK6/Kir1.1f. ROMK6 was nearly identical to ROMK1, but possessed an 122-bp insertion in the 5′ region. Its deduced amino acid sequence was shorter by 19 amino acids than that of ROMK1 in the amino-terminus. Unlike other previously reported ROMK isoforms, ROMK6 mRNA was ubiquitously expressed in various tissues, including kidney, brain, heart, liver, pancreas and skeletal muscle. Xenopus oocytes injected with ROMK6 cRNA expressed a Ba²⁺-sensitive weakly inwardly rectifying K⁺ current. These results indicate that ROMK6 is a novel functional K⁺ channel and might be involved in K+ secretion in various tissues.

【 授权许可】

Unknown