FEBS Letters | |
Cloning and functional expression of a novel isoform of ROMK inwardly rectifying ATP‐dependent K+ channel, ROMK6 (Kir1.1 f) | |
Isomoto, Shojiro1  Matsuzawa, Yuji2  Matsumoto, Shigeto1  Horio, Yoshiyuki1  Takemura-Kameda, Kaoru2  Yamada, Mitsuhiko1  Kurachi, Yoshihisa1  Yamashita, Shizuya2  Kondo, Chikako1  | |
[1] Department of Pharmacology II, Faculty of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565, Japan;Internal Medicine II, Faculty of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565, Japan | |
关键词: Cloning; Inwardly rectifying K+ channel; ROMK channel; Splice variant; Reverse transcription-polymerase chain reaction; Xenopus oocyte; | |
DOI : 10.1016/S0014-5793(96)01302-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We have identified from rat kidney a novel isoform of ROMK/Kir1.1, designated ROMK6/Kir1.1f. ROMK6 was nearly identical to ROMK1, but possessed an 122-bp insertion in the 5′ region. Its deduced amino acid sequence was shorter by 19 amino acids than that of ROMK1 in the amino-terminus. Unlike other previously reported ROMK isoforms, ROMK6 mRNA was ubiquitously expressed in various tissues, including kidney, brain, heart, liver, pancreas and skeletal muscle. Xenopus oocytes injected with ROMK6 cRNA expressed a Ba2+-sensitive weakly inwardly rectifying K+ current. These results indicate that ROMK6 is a novel functional K+ channel and might be involved in K+ secretion in various tissues.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020303674ZK.pdf | 531KB | download |