Cloning, functional expression and tissue distribution of human α 1C‐adrenoceptor splice variants

FEBS Letters

Muramoto, Noriyuki²  Obika, Kenji¹  Yano, Junichi²  Horie, Kuniko¹  Hirasawa, Akira¹  Takagaki, Kazuchika²  Tsujimoto, Gozoh¹  Takei, Yoshinori¹  Shibata, Katsushi¹  Tanaka, Teruo² 

[1] Department of Molecular, Cell Pharmacology, National Children's Medical Research Center, 3-35-31 Taishido, Setagaya-ku, Tokyo 154, Japan;Molecular Biology Department, Research Laboratories, Nippon Shinyaku Co. Ltd., Nishiohji Hachijo, Minami-ku, Kyoto 601, Japan

关键词: α 1-Adrenoceptor;    Splice variant;    Cloning;    Tissue distribution;    [125I]HEAT;    [125I](2-β-(4-hydroxyphenyl)-ethylaminomethyl)-tetralon e;    WB 4101;    2-(2;    6-dimethoxyphenoxyethyl)-amino-methyl-1;    4-benzodioxane;    U-73122;    1-[6-[[17β-3-methoxyestra-1;    3;    5(10)-trien-17-yl]amino]hexyl]-1H-py rrole-2;    5-dione;    CEC;    chlorethylclonidine;    [Ca2+]i;    the intracellular free Ca2+ concentration;    PAGE;    polyacrylamide gel electrophoresis;    RT-PCR;    reverse-transcription polymerase chain reaction;    CHO cells;    Chinese hamster ovary cells;    NE;    norepinephrine;   

DOI  :  10.1016/0014-5793(95)00330-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report the cloning and characterization of two isoforms of human α _1C-adrenoceptor cDNA (α _1C-2, α _C-3). These isoforms are generated by alternative splicing and differ from the clone we previously isolated (α _1C-1) in their length and sequences of the C-terminal domain. Tissue distribution of mRNAs showed that these variants co-express with α _1C-1 in the human heart, liver, cerebellum and cerebrum. Despite the structural differences, functional experiments in transfected CHO cells showed that the three isoforms have similar ligand binding properties, and all couple with phospholipase C/Ca²⁺ signaling pathway.

【 授权许可】

Unknown