Arabidopsis glutathione‐dependent formaldehyde dehydrogenase is an S‐nitrosoglutathione reductase

FEBS Letters

Sakamoto, Atsushi²  Morikawa, Hiromichi²  Ueda, Manami¹ 

[1] Department of Curriculum and Instruction, Faculty of Education, Hiroshima University, 1-1-2 Kagamiyama, Higashi-Hiroshima 739-8523, Japan;Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8526, Japan

关键词: Glutathione-dependent formaldehyde dehydrogenase;    S–Nitrosoglutathione reductase;    Nitrosative stress;    Nitric oxide signaling;    Arabidopsis;    GS-FDH;    glutathione-dependent formaldehyde dehydrogenase;    GSNO;    S-nitrosoglutathione;    GSNOR;    GSNO reductase;    IPTG;    isopropyl 1-thio-β-D-galactoside;    NO;    nitric oxide;    PCR;    polymerase chain reaction;    RNI;    reactive nitrogen intermediate;    SDS–PAGE;    sodium dodecyl sulfate–polyacrylamide gel electrophoresis;    SNO;    S-nitrosothiol;   

DOI  :  10.1016/S0014-5793(02)02414-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

S–Nitrosoglutathione (GSNO), an adduct of nitric oxide (NO) with glutathione, is known as a biological NO reservoir. Heterologous expression in Escherichia coli of a cDNA encoding a glutathione-dependent formaldehyde dehydrogenase of Arabidopsis thaliana showed that the recombinant protein reduces GSNO. The identity of the cDNA was further confirmed by functional complementation of the hypersensitivity to GSNO of a yeast mutant with impaired GSNO metabolism. This is the first demonstration of a plant GSNO reductase, suggesting that plants possess the enzymatic pathway that modulates the bioactivity and toxicity of NO.

【 授权许可】

Unknown