N 6‐Adenine DNA‐methyltransferase in wheat seedlings

FEBS Letters

Fedoreyeva, Larisa I.¹  Vanyushin, Boris F.¹ 

[1] Belozersky Institute of Physical and Chemical Biology, Lomonosov Moscow State University, Moscow 119899, Russia

关键词: Adenine DNA-methyltransferase;    Aging;    Apoptosis;    DNA methylation;    N 6-Methyladenine;    Mitochondrion;    Mitochondrial DNA replication;    Plant;    Wheat;    AdoMet;    S-adenosyl-L-methionine;    mtDNA;    mitochondrial DNA;    SDS;    sodium dodecyl sulfate;    TLC;    thin-layer chromatography;    wadmtase;    wheat N 6-adenine DNA-methyltransferase;   

DOI  :  10.1016/S0014-5793(02)02384-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The N ⁶-adenine DNA-methyltransferase was isolated from the vacuolar vesicle fraction of wheat coleoptiles. In the presence of S-adenosyl-L-methionine the enzyme de novo methylates the first adenine residue in the TGATCA sequence in the single- or double-stranded DNA substrates but it prefers single-stranded structures. Wheat adenine DNA-methyltransferase (wadmtase) is a Mg²⁺- or Ca²⁺-dependent enzyme with a maximum activity at pH 7.5–8.0. Wadmtase seems to be responsible for mitochondrial DNA modification that might be involved in the regulation of replication of mitochondria in plants.

【 授权许可】

Unknown