Recombinant glucocorticoid induced tumor necrosis factor receptor (rGITR) induces NOS in murine macrophage

FEBS Letters

Kwon, Byoung S¹  Shin, Hyun-Hee¹  Choi, Hye-Seon¹  Lee, Yun-Hwa¹  Kim, Suk-Gi¹  Lee, Moo-Hyung¹ 

[1] Department of Biological Sciences and the Immunomodulation Research Center, University of Ulsan, Ulsan 680-749, South Korea

关键词: Glucocorticoid induced tumor necrosis factor receptor;    Inducible nitric oxide synthase;    Macrophage;    Nitric oxide;    GITR;    glucocorticoid induced tumor necrosis factor receptor;    TNFR;    tumor necrosis factor receptor;    iNOS;    inducible nitric oxide synthase;    Ab;    antibody;    IPTG;    isopropyl 1-thio-β-D-galactopyranoside;   

DOI  :  10.1016/S0014-5793(02)02379-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Glucocorticoid induced tumor necrosis factor receptor (GITR) is a new member of the tumor necrosis factor–nerve growth factor receptor superfamily of which the function has not been well studied. The extracellular domain of GITR was produced in Escherichia coli and purified as a single band of predicted M _r of 18.0 kDa. GITR and GITR ligand were expressed constitutively on the surface of Raw 264.7 macrophage cell line and murine peritoneal macrophages. An extracellular domain of GITR can activate murine macrophages to express inducible nitric oxide synthase and to generate nitric oxide in a dose- and time-dependent manner.

【 授权许可】

Unknown