The actin‐binding protein Filamin‐A interacts with the metabotropic glutamate receptor type 7

FEBS Letters

Enz, Ralf¹ 

[1] Institut für Biochemie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Fahrstr. 17, D-91054 Erlangen, Germany

关键词: mGluR;    Filamin-A;    Protein–protein interaction;    Yeast two-hybrid;    Expression pattern;    GST;    glutathione-S-transferase;    HEK-293 cells;    human embryonic kidney cells;    mGluR;    metabotropic glutamate receptor;    PICK1;    protein interacting with C-kinase;    PKC;    protein kinase C;   

DOI  :  10.1016/S0014-5793(02)02361-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A yeast two-hybrid screen identified Filamin-A as a binding partner of the metabotropic glutamate receptor type 7b (mGluR7b) splice variant. In addition, Filamin-A interacted with mGluR4a, mGluR5a, mGluR5b, mGluR7a and mGluR8a. Domain mapping revealed that alternative splicing of mGluR4, mGluR7 and mGluR8 C-termini regulated the interaction. A conserved tyrosine within mGluR C-termini was identified to mediate the binding to Filamin-A. Protein interactions were verified in biochemical assays using recombinant and native proteins. Finally, co-expression of Filamin-A and mGluR7 splice variants was shown in brain regions. These findings suggest that Filamin-A may physically link metabotropic glutamate receptors to the actin cytoskeleton.

【 授权许可】

Unknown