The major light‐harvesting pigment protein of Acaryochloris marina

FEBS Letters

Quinnell, Rosanne G¹  Chen, Min¹  Larkum, Anthony W.D¹ 

[1] School of Biological Sciences, The University of Sydney, Sydney, NSW 2006, Australia

关键词: Chlorophyll d;    Light-harvesting pigment proteins;    Chl a/b protein;    Photosynthesis;    Acaryochloris marina;    Chl;    chlorophyll;    doDM;    dodecyl maltoside;    lhc;    light-harvesting complex;    OG;    octyl glucoside;    PMSF;    phenylmethanesulphonyl fluoride;    PSI;    photosystem I;    PSII;    photosystem II;    ZW14;    zwittergent 3-14;   

DOI  :  10.1016/S0014-5793(02)02315-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The major light-harvesting protein complex containing chlorophyll (Chl) d was isolated from Acaryochloris marina thylakoid membranes. Isolation was achieved by detergent solubilisation followed by separation on 6–40% sucrose gradients using ultracentrifugation. The best Chl d yield (70%) used 0.3% dodecyl maltoside, 0.15% octyl glucoside, 0.05% zwittergent 3-14 with the detergent:total Chl d ratio around 10:1 (w/w). Characterisation of the light-harvesting pigment protein complex (lhc) involved non-denaturing electrophoresis, SDS–PAGE, absorbance and fluorescence spectroscopy. The main polypeptide in the lhc was shown to be ca. 34 kDa and to contain Chl d and Chl a, indicating that the Acaryochloris lhc is similar to that of prochlorophytes. The Chl a level varied with the culture conditions, which is consistent with previous findings.

【 授权许可】

Unknown