Isolation and characterization of biliprotein aggregates from Acaryochloris marina, a Prochloron‐like prokaryote containing mainly chlorophyll d

FEBS Letters

Senger, Horst¹  Mörschel, Erhard¹  Miyachi, Shigetoh³  Miyashita, Hideaki²  Marquardt, Jürgen¹ 

[1] Fachbereich Biologie/Botanik, Philipps-Universität Marburg, Karl-von-Frisch-Strasse, D-35032 Marburg, Germany;Marine Biotechnology Institute, Kamaishi Laboratories, Kamaishi, Iwate 026, Japan;Marine Biotechnology Institute, Bukyo-ku, Tokyo 113, Japan

关键词: Phycobiliprotein;    Allophycocyanin;    Phycocyanin;    Light harvesting antenna;    Chlorophyll d;    Prochlorophyte;    (Acaryochloris marina);    AP;    allophycocyanin;    Chl;    chlorophyll;    PAGE;    polyacrylamide gel electrophoresis;    PBS;    phycobilisome;    PC;    phycocyanin;    PE;    phycoerythrin;    SDS;    sodium dodecyl sulfate;   

DOI  :  10.1016/S0014-5793(97)00631-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phycobiliprotein aggregates were isolated from the prokaryote Acaryochloris marina, containing chlorophyll d as major pigment. In the electron microscope the biliprotein aggregates appear as rod-shaped structures of 26.0×11.3 nm, composed of four ring-shaped subunits 5.8 nm thick and 11.7 nm in diameter. Spectral data indicate that the aggregates contain two types of biliproteins: phycocyanin and an allophycocyanin-type pigment, with very efficient energy transfer from the phycocyanin- to allophycocyanin-type constituent. The chromophore-binding polypeptides of the pigments have apparent molecular masses of 16.2 and 17.4 kDa. They crossreact with antibodies against phycocyanin and allophycocyanin from a red alga.

【 授权许可】

Unknown