The minimal tRNA: unique structure of Ascaris suum mitochondrial tRNASer UCU having a short T arm and lacking the entire D arm

FEBS Letters

Watanabe, Kimitsuna¹  Ohtsuki, Takashi¹  Kawai, Gota¹ 

[1] Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan

关键词: Unusual tRNA;    Mitochondrion;    Nematode;    Nuclear magnetic resonance;    Stable isotope;    Ascaris suum;    BAP;    Escherichia coli alkaline phosphatase;    EF-Tu;    elongation factor Tu;    HMQC;    heteronuclear multiple quantum correlation;    mt;    mitochondrial;    PEG;    polyethylene glycol;    DTT;    dithiothreitol;   

DOI  :  10.1016/S0014-5793(02)02328-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The tertiary structure of Ascaris suum mitochondrial tRNA^Ser _UCU was examined by nuclear magnetic resonance analysis using its transcript, since tRNA^Ser _UCU, lacking the D arm and possessing a truncated T arm, is the shortest of all the known tRNAs. Most basepairs in the proposed secondary structure of tRNA^Ser _UCU were shown to exist, but the connector region comprising the truncated D loop and the extra loop was flexible. This flexibility, would enable adjustment of the mutual distance between the 3′-terminus and the anticodon consistent with that of usual tRNAs. Thus, tRNA^SerUCU appears to function in a similar way to that of usual tRNAs in the ribosome.

【 授权许可】

Unknown