FEBS Letters | |
The minimal tRNA: unique structure of Ascaris suum mitochondrial tRNASer UCU having a short T arm and lacking the entire D arm | |
Watanabe, Kimitsuna1  Ohtsuki, Takashi1  Kawai, Gota1  | |
[1] Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan | |
关键词: Unusual tRNA; Mitochondrion; Nematode; Nuclear magnetic resonance; Stable isotope; Ascaris suum; BAP; Escherichia coli alkaline phosphatase; EF-Tu; elongation factor Tu; HMQC; heteronuclear multiple quantum correlation; mt; mitochondrial; PEG; polyethylene glycol; DTT; dithiothreitol; | |
DOI : 10.1016/S0014-5793(02)02328-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The tertiary structure of Ascaris suum mitochondrial tRNASer UCU was examined by nuclear magnetic resonance analysis using its transcript, since tRNASer UCU, lacking the D arm and possessing a truncated T arm, is the shortest of all the known tRNAs. Most basepairs in the proposed secondary structure of tRNASer UCU were shown to exist, but the connector region comprising the truncated D loop and the extra loop was flexible. This flexibility, would enable adjustment of the mutual distance between the 3′-terminus and the anticodon consistent with that of usual tRNAs. Thus, tRNASerUCU appears to function in a similar way to that of usual tRNAs in the ribosome.
【 授权许可】
Unknown
【 预 览 】
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