NMR studies of flexible peptides in cavities mimicking the synaptic cleft

FEBS Letters

Tancredi, Teodorico²  Pavone, Luca¹  Temussi, Piero Andrea¹  Crescenzi, Orlando¹ 

[1]Dipartimento di Chimica, Università di Napoli Federico II, via Cinthia, I-80126 Naples, Italy

[2]Istituto di Chimica Biomolecolare, CNR, Via Campi Flegrei 34, I-80078 Pozzuoli, Italy

关键词: Acrylamide gel;    Opioid peptide;    Viscosity;    Synapsis;    MNEI;    single chain monellin;    DMSO;    dimethylsulfoxide;    NOESY;    nuclear Overhauser spectroscopy;   

DOI  :  10.1016/S0014-5793(02)02336-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The interaction of neuropeptides with post-synaptic receptors is characterised by a high entropic barrier originating from the combination of nanomolar concentration with low conformer population. The influence of high viscosity environments on conformer distribution can help overcome this difficulty. In an attempt to simulate the physicochemical conditions of the synaptic cleft, ¹⁵N-labelled enkephalin has been studied in polyacrylamide gels swollen by different aqueous solutions in the temperature range 273–293 K. Nuclear Overhauser enhancement spectra in the gel pores are consistent with a conformational selection or a slowing down of internal motions that can favour the interaction of the peptide with the receptor.

【 授权许可】

Unknown