Sucrose is involved in the diazotrophic metabolism of the heterocyst‐forming cyanobacterium Anabaena sp

FEBS Letters

Flores, Enrique¹  Curatti, Leonardo²  Salerno, Graciela² 

[1] Instituto de Bioquı́mica Vegetal y Fotosı́ntesis, Universidad de Sevilla-CSIC, E-41092 Sevilla, Spain;Centro de Investigaciones Biológicas, FIBA, Mar del Plata, Argentina

关键词: Sucrose metabolism;    Cyanobacteria;    Sucrose synthase;    Nitrogen metabolism;    Anabaena sp. PCC 7119;   

DOI  :  10.1016/S0014-5793(02)02283-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Sucrose synthase (SuS) expression was studied in the filamentous, nitrogen-fixing cyanobacterium Anabaena sp. PCC 7119. SuS activity, SusA polypeptide, and susA mRNA levels were lower in cells cultured diazotrophically than in the presence of combined nitrogen. An insertional susA mutant presented a dramatic increase in sucrose levels, whereas the disaccharide was not detectable in a susA overexpressing strain, indicating that SusA is involved in the cleavage of sucrose in vivo. Diazotrophic growth was impaired in the susA overexpressing strain, suggesting a role for sucrose in diazotrophic metabolism and the involvement of SusA in the control of carbon flux in the N₂-fixing filament.

【 授权许可】

Unknown