Induction of mammalian cell death by a plant Bax inhibitor

FEBS Letters

Reed, John C¹  Uchimiya, Hirofumi²  Yu, Li-Hua²  Kawai-Yamada, Maki²  Watanabe, Kaori²  Naito, Mikihiko² 

[1] The Burnham Institute, La Jolla, CA 92037, USA;Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-0032, Japan

关键词: Apoptosis;    AtBI-1;    Bax;    Chromatin condensation;    Caspase inhibitor;    AtBI-1;    Arabidopsis thaliana orthologue of Bax inhibitor-1;    AtBI-1-ΔC;    AtBI-1 with the carboxyl-terminal 14 residues removed;    BI-1;    Bax inhibitor-1;    EGFP;    enhanced green fluorescence protein;    ER;    endoplasmic reticulum;    HA;    hemagglutinin;    OsBI-1;    Oryza sativa orthologue of Bax inhibitor-1;    PCD;    programmed cell death;    SDS;    sodium dodecyl sulfate;    XIAP;    X-linked inhibitor of apoptosis protein;   

DOI  :  10.1016/S0014-5793(02)02230-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Arabidopsis thaliana AtBI-1 is an orthologue of mammalian Bax inhibitor-1 capable of suppressing Bax-induced cell death in yeast as well as mammalian cells. Here we investigated whether or not AtBI-1 suppresses Bax-induced cell death using human fibrosarcoma HT1080 cells. Surprisingly, AtBI-1 did not block Bax-induced cell death, but it triggered apoptotic cell death in mammalian cells. The proapoptotic effect of AtBI-1 was blocked by the X-linked caspase inhibitor XIAP, suggesting that the cell death caused by AtBI-1 is similar to that caused by Bax.

【 授权许可】

Unknown