FEBS Letters | |
Molecular cloning and characterization of Kv6.3, a novel modulatory subunit for voltage‐gated K+ channel Kv2.1 1 | |
Furuichi, Kiyoshi1  Yokoi, Hiromichi1  Matsushime, Hitoshi1  Inamura, Kohei1  Miyake, Akira1  Nozawa, Katsura1  Sano, Yorikata1  Mochizuki, Shinobu1  Kitada, Chika1  | |
[1] Molecular Medicine Laboratories, Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co., Ltd., 21 Miyukigaoka, Tsukuba, Ibaraki 305-8585, Japan | |
关键词: Voltage-gated K+ channel; Kv6.3; Kv2.1; Modulatory subunit; PCR; polymerase chain reaction; RACE; rapid amplification of cDNA ends; RT; reverse transcriptase; EST; expressed sequence tag; GAPDH; glyceraldehyde-3-phosphate dehydrogenase; PBS; phosphate-buffered saline; CDS; coding sequence; | |
DOI : 10.1016/S0014-5793(02)02267-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We report identification and characterization of Kv6.3, a novel member of the voltage-gated K+ channel. Reverse transcriptase-polymerase chain reaction analysis indicated that Kv6.3 was highly expressed in the brain. Electrophysiological studies indicated that homomultimeric Kv6.3 did not yield a functional voltage-gated ion channel. When Kv6.3 and Kv2.1 were co-expressed, the heteromultimeric channels displayed the decreased rate of deactivation compared to the homomultimeric Kv2.1 channels. Immunoprecipitation studies indicated that Kv6.3 bound with Kv2.1 in co-transfected cells. These results indicate that Kv6.3 is a novel member of the voltage-gated K+ channel which functions as a modulatory subunit.
【 授权许可】
Unknown
【 预 览 】
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