Molecular cloning and characterization of Kv6.3, a novel modulatory subunit for voltage‐gated K+ channel Kv2.1 1

FEBS Letters

Furuichi, Kiyoshi¹  Yokoi, Hiromichi¹  Matsushime, Hitoshi¹  Inamura, Kohei¹  Miyake, Akira¹  Nozawa, Katsura¹  Sano, Yorikata¹  Mochizuki, Shinobu¹  Kitada, Chika¹ 

[1]Molecular Medicine Laboratories, Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co., Ltd., 21 Miyukigaoka, Tsukuba, Ibaraki 305-8585, Japan

关键词: Voltage-gated K+ channel;    Kv6.3;    Kv2.1;    Modulatory subunit;    PCR;    polymerase chain reaction;    RACE;    rapid amplification of cDNA ends;    RT;    reverse transcriptase;    EST;    expressed sequence tag;    GAPDH;    glyceraldehyde-3-phosphate dehydrogenase;    PBS;    phosphate-buffered saline;    CDS;    coding sequence;   

DOI  :  10.1016/S0014-5793(02)02267-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report identification and characterization of Kv6.3, a novel member of the voltage-gated K⁺ channel. Reverse transcriptase-polymerase chain reaction analysis indicated that Kv6.3 was highly expressed in the brain. Electrophysiological studies indicated that homomultimeric Kv6.3 did not yield a functional voltage-gated ion channel. When Kv6.3 and Kv2.1 were co-expressed, the heteromultimeric channels displayed the decreased rate of deactivation compared to the homomultimeric Kv2.1 channels. Immunoprecipitation studies indicated that Kv6.3 bound with Kv2.1 in co-transfected cells. These results indicate that Kv6.3 is a novel member of the voltage-gated K⁺ channel which functions as a modulatory subunit.

【 授权许可】

Unknown