Inhibitory Effects of Isoliquiritigenin and Licorice Extract on Voltage-Dependent K+ Currents in H9c2 Cells

Journal of Pharmacological Sciences

Kenji Takahashi⁴  Ryo Maeda⁴  Chisato Noguchi²  Junko Kimura⁴  Masahiro Murakawa²  Jing Yang¹  Kazuho Sakamoto⁴  Tomoyuki Ono⁴  Hiroshi Takasugi³ 

[1] Department of Pharmacology, School of Medicine, Wuhan University, China;Department of Anesthesiology, Fukushima Medical University, School of Medicine, Japan;Drug Research Section, Tokyo Research Laboratories, TOA EIYO Ltd., Japan;Department of Pharmacology, Fukushima Medical University, School of Medicine, Japan

关键词: isoliquiritigenin;    ultra-rapidly activating delayed-rectifier K+ current;    H9c2 cell;    Kv2.1;    Kv1.5;   

DOI  :  10.1254/jphs.08227FP

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(38)Cited-By(7)The effect of isoliquiritigenin (ISL), a component of licorice, on the voltage-dependent, ultra-rapidly activating delayed-rectifier K+ current (IKur) was examined in H9c2 cells, a cell-line derived from rat cardiac myoblasts. IKur was recorded using the whole-cell patch clamp method with a pipette solution containing 140 mM K+. Depolarizing voltage pulses of 200-ms duration were given with 10-mV steps every 10 s from −40 mV holding potential. ISL inhibited IKur in a concentration-dependent manner. The median inhibitory concentration (IC50) of ISL was approximately 0.11 μM and the Hill coefficient was 0.71. Using CHO cells expressing Kv1.5 IKur channels, ISL also inhibited Kv1.5 IKur, but less potently than the IKur current in H9c2 cells. Furthermore, in H9c2 cells, the licorice extract itself inhibited IKur in a manner similar to ISL. We conclude that ISL, one component of licorice, is a potent inhibitor of K+ channels, which specifically in H9c2 cells could be Kv2.1, and that this inhibition may be involved in various pharmacological effects of licorice.

【 授权许可】

Unknown