Identification of functional VEGF receptors on human platelets

FEBS Letters

Selheim, Frode¹  Vassbotn, Flemming S¹  Holmsen, Holm¹ 

[1] Department of Biochemistry and Molecular Biology, University of Bergen, Bergen, Norway

关键词: Vascular endothelial growth factor;    Fms-like tyrosine kinase;    Kinase-insert domain region;    Mitogen-activated protein kinase;    Phosphoinositide 3-kinase;    Flt-1;    fms-like tyrosine kinase;    KDR;    kinase-insert domain region;    MAPK;    mitogen-activated protein kinase;    PDGF;    platelet-derived growth factor;    PI3K;    phosphoinositide 3-kinase;    PtdIns(3;    4;    5)P3;    phosphatidylinostol 3;    4;    5-trisphosphate;    PMSF;    phenylmethylsulfonyl fluoride;    VEGF;    vascular endothelial growth factor;    VEGFR;    vascular endothelial growth factor receptor;   

DOI  :  10.1016/S0014-5793(02)02232-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Platelets secrete platelet-derived growth factor (PDGF) and vascular endothelial growth factor (VEGF) upon stimulation. We have demonstrated that platelets have functionally active PDGF α-receptors, a transmembrane tyrosine kinase involved in negative feedback regulation. Here we demonstrate the presence of the related VEGF receptors fms-like tyrosine kinase-1 and kinase-insert domain region on human platelets. VEGF itself did not cause platelet aggregation. However, addition of exogenous VEGF to SFRLLN or thrombin-stimulated platelets potentiated platelet aggregation. Moreover, thrombin-induced phosphoinositide 3-kinase and mitogen-activated protein kinase activity were enhanced in the presence of VEGF.

【 授权许可】

Unknown