Conversion of L‐tryptophan to serotonin and melatonin in human melanoma cells

FEBS Letters

Semak, Igor⁴  Szczesniewski, Andre¹  Pisarchik, Alexander⁵  Slominski, Andrzej⁵  Wortsman, Jacobo²  Sweatman, Trevor³ 

[1] Agilent Techologies, Schaumburg, IL 60173, USA;Department of Internal Medicine, Southern Illinois University, Springfield, IL 62794, USA;Department of Pharmacology, University of Tennessee Health Science Center, Memphis, TN 38163, USA;Department of Biochemistry, Belarus State University, Minsk 220080, Byelorussia;Department of Pathology, University of Tennessee Health Science Center, 899 Madison Avenue, Room 576M, Memphis, TN 38163, USA

关键词: Melanoma cell;    Serotonin;    Melatonin;    N-Acetylserotonin;   

DOI  :  10.1016/S0014-5793(01)03319-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We showed in human melanoma cells tryptophan hydroxylase (TPH) and hydroxyindole methyltransferase genes expression with the sequential enzymatic activities of TPH, serotonin (Ser) N-acetyltransferase and hydroxyindole methyltransferase. The presence of the products Ser, 5OH-tryptophan, N-acetylserotonin, melatonin (Mel), 5-methoxytryptamine and 5-methoxytryptophol was documented by liquid chromatography–mass spectrometry. Thus, human melanoma cells can synthesize and metabolize Ser and Mel.

【 授权许可】

Unknown