Progastrin processing differs in 7B2 and PC2 knockout animals: a role for 7B2 independent of action on PC2

FEBS Letters

Lindberg, Iris²  Rehfeld, Jens F.¹  Friis-Hansen, Lennart¹ 

[1] Department of Clinical Biochemistry, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark;Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, New Orleans, LA, USA

关键词: Chaperone;    Gastrin;    Progastrin;    Prohormone convertase;    Prohormone processing;    7B2;    CPB;    carboxypeptidase B;    PAM;    peptidyl-glycine α-amidating monooxygenase;    PC;    prohormone convertase;    POMC;    proopiomelanocortin;    RIA;    radioimmunoassay;   

DOI  :  10.1016/S0014-5793(01)03234-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cellular synthesis of neuroendocrine peptides requires prohormone convertases (PCs). In order to determine the role of PC2 for gastrin synthesis, we examined antral extracts from mice lacking PC2 or its chaperone, 7B2. The overall concentrations of precursors and α-amidated gastrins were similar in all mice. Chromatography, however, revealed that while the K₅₃–K₅₄ site was almost fully cleaved in controls and half cleaved in PC2 null mice, only 23% was cleaved in 7B2 null mice. The results show that PC2 and 7B2 both are required for synthesis of the main form of gastrin (gastrin-17), and that 7B2 exhibits effects beyond PC2-mediated cleavages.

【 授权许可】

Unknown