Isolation and characterization of the intact gastrin precursor from a gastrinoma

FEBS Letters

Pauwels, S.¹  Desmond, H.³  Gregory, H.²  Dockray, G.J.³  Varro, A.³  Young, Janice² 

[1] Department of Nuclear Medicine, University of Louvain, Medical School, Brussels, Belgium;Imperial Chemical Industries PLC, Pharmaceuticals Division, Macclesfield, England;MRC Secretory Control Group, Physiological Laboratory, University of Liverpool, Liverpool, England

关键词: Progastrin;    Hormone synthesis;    Amino acid sequence;   

DOI  :  10.1016/0014-5793(87)81334-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Antibodies to the extreme C-terminal region of human progastrin have been used to monitor the isolation of high-M _r immunoreactive material in a gastrinoma extract. Microsequence analysis of the product revealed amino acid residues in the first 18 positions corresponding to those predicted from the cDNA sequence for preprogastrin starting at position 22; the sequence and immunochemical data together allow the identification of this material as intact progastrin. Implications for gastrin biosynthesis are discussed.

【 授权许可】

Unknown