Reactive oxygen species generated from the mitochondrial electron transport chain induce cytochrome c dissociation from beef‐heart submitochondrial particles via cardiolipin peroxidation. Possible role in the apoptosis

FEBS Letters

Pistolese, Marilva¹  Paradies, Giuseppe¹  Petrosillo, Giuseppe¹  Ruggiero, Francesca Maria¹ 

[1] Department of Biochemistry and Molecular Biology and C.N.R Unit for the Study of Mitochondria and Bioenergetics, University of Bari, Via E, Orabona 4, 70126, Bari, Italy

关键词: Reactive oxygen species;    Cytochrome c;    Cardiolipin;    Apoptosis;   

DOI  :  10.1016/S0014-5793(01)03206-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cytochrome c release from mitochondria is a critical event in the apoptosis induction. Dissociation of cytochrome c from the mitochondrial inner membrane (IMM) is a necessary first step for cytochrome c release. In the present study, the effect of reactive oxygen species (ROS) on the dissociation of cytochrome c from beef-heart submitochondrial particles (SMP) and on the cardiolipin content was investigated. Exposure of SMP to mitochondrial-mediated ROS generation resulted in a large dissociation of cytochrome c from SMP and in a parallel loss of cardiolipin. Both these effects were directly and significantly correlated and also abolished by superoxide dismutase+catalase. These results demonstrate that ROS generation induces the dissociation of cytochrome c from IMM via cardiolipin peroxidation. The data may prove useful in clarifying the molecular mechanism underlying the release of cytochrome c from the mitochondria to the cytosol.

【 授权许可】

Unknown