Cytosolic thioredoxin peroxidase I is essential for the antioxidant defense of yeast with dysfunctional mitochondria

FEBS Letters

Pereira, Gonçalo A.G.³  Demasi, Ana P.D.²  Netto, Luis E.S.¹ 

[1] Departamento de Biologia, IB, USP, 05508-900 São Paulo, SP, Brazil;Departamento de Bioquı́mica, IB, UNICAMP, 13083-970 Campinas, SP, Brazil;Departamento de Genética e Evolução, IB, UNICAMP, 13083-970 Campinas, SP, Brazil

关键词: Thioredoxin peroxidase;    Oxidative stress;    Gene expression;    Mitochondrion;    Respiratory deficiency;    ROS;    reactive oxygen species;    cTPx I;    cytosolic thioredoxin peroxidase I;    TSA;    thiol-specific antioxidant;    HRP;    horseradish peroxidase;   

DOI  :  10.1016/S0014-5793(01)03215-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The specific role of cytosolic thioredoxin peroxidase I (cTPx I), encoded by TSA1 (thiol-specific antioxidant), was investigated in the oxidative stress response of Saccharomyces cerevisiae. In most cases, deletion of TSA1 has showed only a slight effect on hydrogen peroxide sensitivity. However, when the functional state of the mitochondria was compromised, the necessity of TSA1 in cell protection against this oxidant was much more evident. All the procedures used to disrupt the mitochondrial respiratory chain promoted increases in the generation of H₂O₂ in cells, which could be related to their elevated sensitivity to oxidative stress. In fact, TSA1 is highly expressed when cells with respiratory deficiency are exposed to H₂O₂. In conclusion, our results indicate that cTPx I is a key component of the antioxidant defense in respiratory-deficient cells.

【 授权许可】

Unknown