Choline containing metabolites during cell transfection: an insight into magnetic resonance spectroscopy detectable changes

FEBS Letters

Nicholson, J.K³  Mann, C.J²  Griffin, J.L³  Scott, J¹  Shoulders, C.C² 

[1] Imperial College Genetics and Genomics Research Institute, Imperial College of Science, Technology and Medicine, Hammersmith Hospital, DuCane Road, London W12 ONN, UK;Genomic and Molecular Medicine Group, MRC Clinical Sciences Centre, Imperial College of Science, Technology and Medicine, Hammersmith Hospital, DuCane Road, London W12 ONN, UK;Biological Chemistry, Biomedical Sciences, Imperial College of Science, Technology and Medicine, Exhibition Road, South Kensington, London SW7 2AZ, UK

关键词: High resolution magic angle spinning;    Metabolic profiling;    Metabolomics;    Phosphocholine;   

DOI  :  10.1016/S0014-5793(01)03175-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Increases in choline containing metabolites have been associated with a number of disorders, including malignant cell growth. In this study, high resolution magic angle spinning ¹H nuclear magnetic resonance spectroscopy was employed to monitor metabolite changes during cell transfection, and an increase in phosphocholine was detected. This increase appears to be correlated with cell membrane disruption associated with the insertion of plasmid DNA into cells, since the level of phosphocholine in mock transfected cells was comparable to that of control cells. These data suggest choline containing metabolite changes detected in vivo using magnetic resonance spectroscopy relate to cell membrane disruption.

【 授权许可】

Unknown