Human monkeypox and smallpox viruses: genomic comparison

FEBS Letters

Totmenin, Alexei V.²  Gutorov, Valery V.²  Petrov, Nikolai A.²  Uvarova, Elena A.²  Mikheev, Maxim V.²  Sisler, Jerry R.⁴  Sandakhchiev, Lev S.²  Babkin, Igor V.²  Jahrling, Peter B.¹  Ryazankina, Olga I.²  Shchelkunov, Sergei N.²  Esposito, Joseph J.³  Safronov, Pavel F.²  Moss, Bernard⁴ 

[1] Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD 21702, USA;State Research Center of Virology and Biotechnology ‘Vector’, Koltsovo, Novosibirsk region 630559, Russia;Centers for Disease Control and Prevention, Atlanta, GA 30333, USA;National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, USA

关键词: Monkeypox virus;    Smallpox virus;    Genome;    Virulence factor;    Ankyrin-like protein;    MPV;    monkeypox virus;    MPV-ZAI;    MPV-CNG;    monkeypox virus strains Zaire-96-I-16 and Congo-8;    VAR;    variola virus;    VAR-IND;    VAR-BSH;    VAR-GAR;    variola virus strains India-1967;    Bangladesh-1975;    Garcia-1966;    VAC;    vaccinia virus;    VAC-COP;    vaccinia virus strain Copenhagen;    CPV;    cowpox virus;    CPV-GRI;    cowpox virus strain GRI-90;    PKR;    double-stranded RNA-dependent protein kinase;    ORF;    open reading frame;    IFN;    interferon;    IL;    interleukin;    SPI;    serine protease inhibitor;   

DOI  :  10.1016/S0014-5793(01)03144-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Monkeypox virus (MPV) causes a human disease which resembles smallpox but with a lower person-to-person transmission rate. To determine the genetic relationship between the orthopoxviruses causing these two diseases, we sequenced the 197-kb genome of MPV isolated from a patient during a large human monkeypox outbreak in Zaire in 1996. The nucleotide sequence within the central region of the MPV genome, which encodes essential enzymes and structural proteins, was 96.3% identical with that of variola (smallpox) virus (VAR). In contrast, there were considerable differences between MPV and VAR in the regions encoding virulence and host-range factors near the ends of the genome. Our data indicate that MPV is not the direct ancestor of VAR and is unlikely to naturally acquire all properties of VAR.

【 授权许可】

Unknown