A role for N‐WASP in invasin‐promoted internalisation

FEBS Letters

McGee, Karen¹  Way, Michael²  Zettl, Markus²  Fällman, Maria¹ 

[1] Department of Microbiology, Umeå University, 901 87 Umeå, Sweden;Cell Motility Laboratory, Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London WC2A 3PX, UK

关键词: Invasin;    Internalization;    N-WASP;    Rac;    Src;    Yersinia;   

DOI  :  10.1016/S0014-5793(01)03139-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phagocytosis of Yersinia pseudotuberculosis occurs through interaction of the bacterial protein invasin with β1-integrins. Here we report that N-WASP plays a role in internalisation of an invasin-expressing, avirulent strain of Y. pseudotuberculosis. Ectopic expression of N-WASP mutants, which affect recruitment of the Arp2/3 complex to the phagosome, reduces uptake of Yersinia. In addition, expression of the Cdc42/Rac-binding (CRIB) region of N-WASP has an inhibitory effect on uptake. Using GFP-tagged Rho GTPase mutants, we provide evidence that Rac1, but not Cdc42, is important for internalisation. Furthermore, activated Rac1 rescues Toxin B, CRIB and Src family kinase inhibitor PP2-mediated impairment of uptake. Our observations indicate that invasin-mediated phagocytosis occurs via a Src and WASP family-dependent mechanism(s), involving the Arp2/3 complex and Rac, but does not require Cdc42.

【 授权许可】

Unknown