Characterization of the AfaD‐like family of invasins encoded by pathogenic Escherichia coli associated with intestinal and extra‐intestinal infections

FEBS Letters

Garcia, Marie-Isabelle¹  Gounon, Pierre³  Nataro, James P.²  Le Bouguénec, Chantal¹  Jouve, Mabel¹ 

[1] Unité de Pathogénie Bactérienne des Muqueuses, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France;Center for Vaccine Development, University of Maryland School of Medicine, 10 S Pine Street, Baltimore, MD 21201, USA;Station Centrale de Microscopie Electronique, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France

关键词: AfaD;    Invasin;    Diffusely-adherent Escherichia coli;    Enteroaggregative Escherichia coli;    Urinary tract infection;    Diarrhea;   

DOI  :  10.1016/S0014-5793(00)01898-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The afimbrial adhesive sheath, encoded by the afa-3 gene cluster, is composed of two proteins with different roles in bacterium–HeLa cell interactions. AfaE is required for adhesion and AfaD for internalization. In this study, we found that the AfaD invasin was structurally and functionally conserved among human afa-expressing strains, independently of AfaE subtype and clinical origin of the Escherichia coli isolate. The AggB protein from enteroaggregative E. coli was also found to be an AfaD-related invasin. These data suggest that AfaD is the prototype of a family of invasins encoded by adhesion-associated operons in pathogenic E. coli.

【 授权许可】

Unknown