Induction of H2O2 synthesis by β‐glucan elicitors in soybean is independent of cytosolic calcium transients

FEBS Letters

Ebel, Chantal¹  Bhagwat, Arvind A²  Ebel, Jürgen⁴  Daxberger, Andrea⁴  Fliegmann, Judith⁴  Mithöfer, Axel⁴  Neuhaus-Url, Gabriele¹  Keister, Donald L³ 

[1] Friedrich Miescher-Institut, CH-4002 Basel, Switzerland;Product Quality and Safety Laboratory, USDA-ARS, BARC-W, Beltsville, MD 20705, USA;Soybean Genomics and Improvement Laboratory, USDA-ARS, BARC-W, Beltsville, MD 20705, USA;Botanisches Institut, Menzinger Str. 67, D-80638 Munich, Germany

关键词: Cytosolic calcium;    β-Glucan;    Plant defence;    Soybean;   

DOI  :  10.1016/S0014-5793(01)03054-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Soybean cell suspension cultures have been used to investigate the role of the elevation of the cytosolic Ca²⁺ concentration in β-glucan elicitors-induced defence responses, such as H₂O₂ and phytoalexin production. The intracellular Ca²⁺ concentration was monitored in transgenic cells expressing the Ca²⁺-sensing aequorin. Two lines of evidence showed that a transient increase of the cytosolic Ca²⁺ concentration is not necessarily involved in the induction of H₂O₂ generation: (i) a Bradyrhizobium japonicum cyclic β-glucan induced the H₂O₂ burst without increasing the cytosolic Ca²⁺ concentration; (ii) two ion channel blockers (anthracene-9-carboxylate, A9C; 5-nitro-2-(3-phenylpropylamino)-benzoate, NPPB) could not prevent a Phytophthora soja β-glucan elicitor-induced H₂O₂ synthesis but did prevent a cytosolic Ca²⁺ concentration increase. Moreover, A9C and NPPB inhibited P. sojae β-glucan-elicited defence-related gene inductions as well as the inducible accumulation of phytoalexins, suggesting that the P. sojae β-glucan-induced transient cytosolic Ca²⁺ increase is not necessary for the elicitation of H₂O₂ production but is very likely required for phytoalexin synthesis

【 授权许可】

Unknown