Phosphoenolpyruvate carboxylase kinase involved in C4 photosynthesis in Flaveria trinervia: cDNA cloning and characterization1

FEBS Letters

Furumoto, Tsuyoshi¹  Izui, Katsura¹  Tsuchida, Yuhei²  Hata, Shingo¹  Izumida, Atsushi² 

[1] Laboratory of Plant Physiology, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan;Laboratory of Plant Physiology, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan

关键词: C4 photosynthesis;    Phosphoenolpyruvate carboxylase;    Phosphoenolpyruvate carboxylase kinase;    Regulatory phosphorylation;    Flaveria trinervia;    CAM;    Crassulacean acid metabolism;    CaMK;    calmodulin-dependent protein kinase;    CDPK;    calcium-dependent protein kinase;    DTT;    dithiothreitol;    GSSG;    oxidized-form glutathione;    PEPC;    phosphoenolpyruvate carboxylase;    PEPC-PK;    phosphoenolpyruvate carboxylase kinase;   

DOI  :  10.1016/S0014-5793(01)02994-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In C₄ plants, phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31), a key enzyme in C₄ photosynthesis, is controlled by reversible phosphorylation of a conserved Ser residue near the N-terminus. We now report the first cloning of a cDNA from a C₄ plant, Flaveria trinervia, which encodes the specific protein kinase (FtPEPC-PK) involved in the phosphorylation of C₄-form PEPC. Several lines of supportive evidence are: strict substrate specificity of the recombinant enzyme, prominent light/dark response of the transcript level and abundant expression in leaves of C₄ plant (F. trinervia) but very low expression in a C₃ plant of the same genus (Flaveria pringlei). We also discuss the possibility that the FtPEPC-PK gene has co-evolved with the PEPC gene to participate in C₄ photosynthesis.

【 授权许可】

Unknown