Dynamic flexibility in the Escherichia coli genome

FEBS Letters

Tsai, Lu¹  Sun, Zhirong¹ 

[1] Department of Biological Science and Technology, Tsinghua University, Beijing 100084, PR China

关键词: Base-pair step interaction;    DNA flexibility;    Statistical mechanical model;    Whole-genome analysis;    NDB;    nucleic acid database;    CDS;    coding region;   

DOI  :  10.1016/S0014-5793(01)02978-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Empirical rules based on tetranucleotide parameters were presented to predict the structural parameters twist (Ω), roll (ρ), tilt (τ) and slide (D_y ). A statistical mechanical model was used to analyze the flexibility of the Escherichia coli genome. The replication terminus region displayed a low level of flexibility. A strong correlation can be seen between G+C content and flexibility. Average flexibilities in the coding regions were found to be significantly larger than those in non-coding regions. The flexible characteristics in the 5′-neighborhood of the coding regions and in three class sigma promoter sequences in the E. coli genome were also analyzed.

【 授权许可】

Unknown