In vitro transactivation of Bacillus subtilis RNase P RNA

FEBS Letters

Kim, Hadong¹  Leeper, Thomas C¹  Poelling, Richard R¹  Meyer, Melissa A¹  Schmidt, Francis J¹ 

[1] Department of Biochemistry, M121 Medical Sciences, University of Missouri-Columbia, Columbia, MO 65212, USA

关键词: Catalytic RNA;    RNA–RNA interaction;    Kinetics;    Phylogenetic variation;    RNA world;   

DOI  :  10.1016/S0014-5793(01)02929-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Deletion of the ‘signature’ PL5.1 stem-loop structure of a Type II RNase P RNA diminished its catalytic activity. Addition of PL5.1 in trans increased catalytic efficiency (k _cat/K _M) rather than k _cat. Transactivation was due to the binding of a single PL5.1 species per ribozyme with an apparent K _d near 600 nM. The results are consistent with the role of PL5.1 being to position the substrate near the active site of the ribozyme, and with the hypothesis that ribozymes can evolve by accretion of preformed smaller structures.

【 授权许可】

Unknown