Different target‐site specificities of the hairpin ribozyme in cis and trans cleavages

FEBS Letters

Sasaki-Tozawa, Noriko¹  Fujitani, Kazuko¹  Kikuchi, Yo¹ 

[1] Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, Machida-shi, Tokyo 194, Japan

关键词: Catalytic RNA;    Satellite RNA;    Tobacco ringspot virus;    Arabis mosaic virus;    Chicory yellow mottle virus;   

DOI  :  10.1016/0014-5793(93)80316-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The hairpin ribozyme cleaves a phosphodiester bond at the 5' side of a 5'GUC3' sequence of an RNA with high efficiency. An RNA having a 5'GUA3' sequence instead of the GUC sequence is a poor substrate for this ribozyme. Here, we show that this is indeed so in a trans-acting ribozyme system, but in a cis-acting ribozyme system this ribozyme cleaves the 5' side of a GUA sequence as efficiently as the wild-type cleaves the GUC sequence. One base substitution in the ribozyme also affected the target-site specificity in the cis-acting system.

【 授权许可】

Unknown