期刊论文详细信息
FEBS Letters | |
Different target‐site specificities of the hairpin ribozyme in cis and trans cleavages | |
Sasaki-Tozawa, Noriko1  Fujitani, Kazuko1  Kikuchi, Yo1  | |
[1] Mitsubishi Kasei Institute of Life Sciences, 11 Minamiooya, Machida-shi, Tokyo 194, Japan | |
关键词: Catalytic RNA; Satellite RNA; Tobacco ringspot virus; Arabis mosaic virus; Chicory yellow mottle virus; | |
DOI : 10.1016/0014-5793(93)80316-M | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The hairpin ribozyme cleaves a phosphodiester bond at the 5' side of a 5'GUC3' sequence of an RNA with high efficiency. An RNA having a 5'GUA3' sequence instead of the GUC sequence is a poor substrate for this ribozyme. Here, we show that this is indeed so in a trans-acting ribozyme system, but in a cis-acting ribozyme system this ribozyme cleaves the 5' side of a GUA sequence as efficiently as the wild-type cleaves the GUC sequence. One base substitution in the ribozyme also affected the target-site specificity in the cis-acting system.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020298483ZK.pdf | 413KB | download |