Localization of neutral ceramidase in caveolin‐enriched light membranes of murine endothelial cells

FEBS Letters

Meacci, Elisabetta²  Farnararo, Marta²  Bruni, Paola²  Romiti, Elena²  Ito, Makoto¹  Becciolini, Laura²  Mitsutake, Susumu¹  Tanzi, Giuditta² 

[1] Department of Bioscience and Biotechnology, Graduate School Kyushu University, Fukuoka, Japan;Dipartimento di Scienze Biochimiche, Viale G.B. Morgagni 50, 50134 Florence, Italy

关键词: Neutral ceramidase;    Caveolin-enriched light membrane;    Cholesterol;    Murine endothelial cell;    SMase;    sphingomyelinase;    SM;    sphingomyelin;    Cer;    ceramide;    CDase;    ceramidase;    Sph;    sphingosine;    cav;    caveolin;    CELM;    caveolin-enriched light membrane;    IM;    intermediate density membrane;    HM;    high density membrane;    CD;    cyclodextrin;   

DOI  :  10.1016/S0014-5793(01)02878-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Sphingomyelinase (SMase) and ceramidase (CDase) activities participate in sphingomyelin (SM) metabolism and have a role in the signal transduction of a variety of ligands. In this study evidence is presented that caveolin-enriched light membranes (CELMs) of murine endothelial cells, characterized by high SM, ceramide (Cer) and cholesterol content, bear acid and neutral SMase as well as neutral CDase activities. Localization of neutral CDase in CELMs was confirmed by Western analysis. Notably, cell treatment with cyclodextrin, which depleted cell cholesterol, did not affect acid or neutral SMase activities but significantly enhanced neutral CDase activity in CELMs, indicating a negative role for cholesterol in CDase regulation. These findings suggest that neutral CDase is implicated, together with SMase activities, in the control of caveolar Cer content that may be critical for caveola dynamics.

【 授权许可】

Unknown