Toxicity of the bacterial luciferase substrate, n‐decyl aldehyde, to Saccharomyces cerevisiae and Caenorhabditis elegans

FEBS Letters

Glover, L.A.¹  Porter, A.J.R.¹  Paton, G.I.²  Lagido, C.¹  Killham, K.²  Hollis, R.P.¹  Pettitt, J.¹ 

[1] Department of Molecular and Cell Biology, University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen AB25 2ZD, UK;Department of Plant and Soil Science, University of Aberdeen, Cruickshank Building, St. Machar Drive, Aberdeen AB24 3UU, UK

关键词: Biosensor;    Luciferase;    Bioluminescence;    Toxicity;   

DOI  :  10.1016/S0014-5793(01)02905-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

This study determined that the bacterial luciferase fusion gene (luxAB) was not a suitable in vivo gene reporter in the model eukaryotic organisms Saccharomyces cerevisiae and Caenorhabditis elegans. LuxAB expressing S. cerevisiae strains displayed distinctive rapid decays in luminescence upon addition of the bacterial luciferase substrate, n-decyl aldehyde, suggesting a toxic response. Growth studies and toxicity bioassays have subsequently confirmed, that the aldehyde substrate was toxic to both organisms at concentrations well tolerated by Escherichia coli. As the addition of aldehyde is an integral part of the bacterial luciferase activity assay, our results do not support the use of lux reporter genes for in vivo analyses in these model eukaryotic organisms.

【 授权许可】

Unknown