Direct observation of membrane retrieval in chromaffin cells by capacitance measurements

FEBS Letters

Henkel, Andreas W²  Henkel, M.Kerstin²  Horstmann, Heinz¹ 

[1] Institute of Molecular and Cell Biology, The National University of Singapore, 30 Medical Drive, Singapore 117609, Singapore;Department of Psychiatry (Molecular Neurobiology), University of Erlangen, Schwabachanlage 6-10, 91054 Erlangen, Germany

关键词: Endocytosis;    Chromaffin cell;    Clathrin-coated vesicle;    Capacitance measurement;    Immuno-electron microscopy;    LDCVs;    large;    dense core vesicles;    HRP;    horseradish peroxidase;   

DOI  :  10.1016/S0014-5793(01)02861-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

This study was focussed on the identification of the endocytic organelles in chromaffin cells which retrieve large, dense core vesicle (LDCV)-membrane components from the plasma membrane. For this purpose, ‘on-cell’ capacitance measurements and electron microscopy were employed. We found capacitance steps and capacitance flickers, corresponding to single exo- and endocytic events. The analysis revealed that the total membrane surface of completely fused LDCVs is recycled by large endocytic vesicles and smaller, most likely clathrin-coated vesicles, at approximately the same ratio. These results were confirmed by rapid-freeze immuno-electron microscopy, where an extracellular marker was rapidly internalized into endocytic vesicles that morphologically resembled LDCVs.

【 授权许可】

Unknown